M.B.B.S. EXAMINATION BIOCHEMISTRY PAPER-II

A 15 year old boy complained of swelling and pain in the distal phalangeal joints.  Blood investigation showed the following results.

Blood urea             : 15 mg%

Serum uric acid      :  16 mg%

On diagnosing the pathology, the physician decided to treat patient with allopurinol

i) What is your probable diagnosis in the above patient?

ii) Which 2 other blood investigations would you suggest?

iii)Comment on the serum uric acid level and explain the

    cause of pain in joints.

iv) What is the biochemical explanation for the treatment

    given in the above patient?

1+1+4+4=10

Discuss the metabolism of glycine under the following headings.

i) Synthesis   ii)  Catabolism 

 iii) Specialized compounds synthesized

3+3+4=10

WRITE SHORT NOTES ON:

5x4=20

Explain the absorption, transport and storage of dietary iron in the body.

Give an account of post transcription modifications

Describe PCR with diagram and list out two uses of it.

Compare and contrast (1 similarity and 2 differences) DNA polymerase and RNA polymerase

Compare and contrast (1 similarity and 2 differences) prehepatic and post hepatic jaundice

WRITE BRIEFLY ON:

5x2=10

Azaserine is used as an anticancer agent

Wilson’s disease

Oxalates in diet inhibit absorption of iron and calcium

Metabolic acidosis

Serum alpha fetoprotein and carcinoembryonic antigen